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Int. J. Mol. Sci. 2016, 17(4), 511; doi:10.3390/ijms17040511

The Effects of Different Purifying Methods on the Chemical Properties, in Vitro Anti-Tumor and Immunomodulatory Activities of Abrus cantoniensis Polysaccharide Fractions

1
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
2
Centre for Food Research and Innovation, Lincoln University, Lincoln 7648, New Zealand
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 15 March 2016 / Revised: 29 March 2016 / Accepted: 31 March 2016 / Published: 6 April 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [5722 KB, uploaded 6 April 2016]   |  

Abstract

Abrus cantoniensis (Hance) is a popular Chinese vegetable consumed as a beverage, soup or folk medicine. To fully exploit the potential of the polysaccharide in Abrus cantoniensis, nine polysaccharide fractions of Abrus cantoniensis were isolated and purified (AP-AOH30-1, AP-AOH30-2, AP-AOH80-1, AP-AOH80-2, AP-ACl-1, AP-ACl-2, AP-ACl-3, AP-H and AP-L). Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography (GC) were used to characterize these Abrus polysaccharides fractions (APF). In vitro anti-tumor and immunomodulatory activities were also investigated and compared using the rank-sum ratio (RSR) method. Results demonstrated significant differences in the structure and bioactivities among APF, which were associated to the process used for their purification. Among the APF, AP-ACl-3 yield was 613.5 mg/kg of product and consisted of rhamnose (9.8%), arabinose (8.9%), fructose (3.0%), galactose (9.9%), glucose (4.3%), galacturonic acid (3.0%) and glucuronic acid (61.1%) with a molecular weight of 4.4 × 104 Da. Furthermore, AP-ACl-3 exhibited considerable bioactivities significantly preventing the migration of MCF-7 cells and stimulating lymphocyte proliferation along with nitric oxide (NO) production of peritoneal macrophages. AP-ACl-3 could be explored as a novel potential anti-tumor and immunomodulatory agent. View Full-Text
Keywords: polysaccharide; Abrus cantoniensis; purifying method; anti-tumor; immunomodulatory polysaccharide; Abrus cantoniensis; purifying method; anti-tumor; immunomodulatory
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Wu, S.; Fu, X.; Brennan, M.A.; Brennan, C.S.; Chun, C. The Effects of Different Purifying Methods on the Chemical Properties, in Vitro Anti-Tumor and Immunomodulatory Activities of Abrus cantoniensis Polysaccharide Fractions. Int. J. Mol. Sci. 2016, 17, 511.

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