Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed
AbstractLesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production. View Full-Text
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Chen, G.Q.; van Erp, H.; Martin-Moreno, J.; Johnson, K.; Morales, E.; Browse, J.; Eastmond, P.J.; Lin, J.-T. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. Int. J. Mol. Sci. 2016, 17, 507.
Chen GQ, van Erp H, Martin-Moreno J, Johnson K, Morales E, Browse J, Eastmond PJ, Lin J-T. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. International Journal of Molecular Sciences. 2016; 17(4):507.Chicago/Turabian Style
Chen, Grace Q.; van Erp, Harrie; Martin-Moreno, Jose; Johnson, Kumiko; Morales, Eva; Browse, John; Eastmond, Peter J.; Lin, Jiann-Tsyh. 2016. "Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed." Int. J. Mol. Sci. 17, no. 4: 507.
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