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Int. J. Mol. Sci. 2016, 17(4), 507; doi:10.3390/ijms17040507

Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed

1
Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA
2
Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA
3
Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Ute Roessner
Received: 4 March 2016 / Revised: 25 March 2016 / Accepted: 30 March 2016 / Published: 6 April 2016
(This article belongs to the Special Issue Metabolomics in the Plant Sciences)
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Abstract

Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production. View Full-Text
Keywords: lesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering lesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chen, G.Q.; van Erp, H.; Martin-Moreno, J.; Johnson, K.; Morales, E.; Browse, J.; Eastmond, P.J.; Lin, J.-T. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. Int. J. Mol. Sci. 2016, 17, 507.

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