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Int. J. Mol. Sci. 2016, 17(4), 496; doi:10.3390/ijms17040496

Characterization of Virulence Properties of Aeromonas veronii Isolated from Diseased Gibel Carp (Carassius gibelio)

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
College of Ocean, Huaihai Institute of Technology, Lianyungang 222005, China
3
Bren School of Environmental Science and Management, University of California, Santa Barbara, CA 93106, USA
4
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Jun Li
Received: 13 February 2016 / Revised: 16 March 2016 / Accepted: 29 March 2016 / Published: 1 April 2016
(This article belongs to the Special Issue Fish Molecular Biology)
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Abstract

Aeromonas veronii is a kind of opportunistic pathogen to fish and humans, significantly impending aquaculture production. Recently, we isolated two A. veronii strains, named GYC1 and GYC2, from diseased Gibel carp (Carassius gibelio) in China. Based on gyrB (DNA gyrase B subunit) genes of GYC1 and GYC2, the constructed phylogenetic tree showed that the two strains were clustered with A. veronii. Sixteen virulence genes related to the pathogenicity of Aeromonas spp. were subjected to PCR assay. The genes of ompAI, ompAII, lafA, act, aer, fla, gcaT and acg were detected in the two strains, while genes of hly, ahp, lip, ast and alt were not detected. Additionally, genes eprCAI, ela and exu were only detected in the strain GYC1. Furthermore, the results of extracellular enzyme analysis revealed that the two isolates can produce hemolysin, caseinase, esterase, amylase and lecithinase, which were closely related to the pathogenicity of the two strains. However, the results showed that there was no gelatinase activity in either strain. According to the antibiotic resistant assay, the two strains were sensitive to cephalosporins and aminoglycosides, while they were resistant to penicillins and quinolones. Through this study, the virulence characteristics, including virulence genes and extracellular enzymes, the pathogenicity of A. veronii was clarified, enhancing the understanding about this pathogenic bacterium and providing the theoretical basis in disease control. View Full-Text
Keywords: Aeromonas veronii; Carassius gibelio; virulence gene; proteinase; antibiotic resistance Aeromonas veronii; Carassius gibelio; virulence gene; proteinase; antibiotic resistance
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Sun, J.; Zhang, X.; Gao, X.; Jiang, Q.; Wen, Y.; Lin, L. Characterization of Virulence Properties of Aeromonas veronii Isolated from Diseased Gibel Carp (Carassius gibelio). Int. J. Mol. Sci. 2016, 17, 496.

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