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Int. J. Mol. Sci. 2016, 17(3), 357; doi:10.3390/ijms17030357

Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
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Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 5 February 2016 / Revised: 28 February 2016 / Accepted: 4 March 2016 / Published: 10 March 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Background: Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT). Methods: Rats were treated with EA once per day for eight consecutive days, and their L4-5 spinal cords were collected at days 0, 2, 4, 6 and 8. The levels of three spinal GTs and their mRNAs were detected with Western blot and pPCR, respectively. Then, riluzole, a positive GT regulator, was administered intrathecally in order to observe its effect on EA analgesia after repeated EA. Results: The expression levels of the spinal GTs increased at days 2 and 4, and gradually decreased as the times of EA increased. At day 8, no difference was observed in the spinal GTs between the sham treatment and the EA treatment. Intrathecal administration of riluzole dose-dependently attenuated the decreased EA analgesia. Conclusion: These results indicated the participation of the spinal GTs in EAT. View Full-Text
Keywords: electroacupuncture tolerance; GLAST; GLT-1; EAAC1; riluzole; tail flick latency; rat electroacupuncture tolerance; GLAST; GLT-1; EAAC1; riluzole; tail flick latency; rat
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MDPI and ACS Style

Cui, L.; Ding, Y.; Zeng, J.; Feng, Y.; Li, M.; Ding, M. Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance. Int. J. Mol. Sci. 2016, 17, 357.

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