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Int. J. Mol. Sci. 2016, 17(2), 231; doi:10.3390/ijms17020231

Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress

Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan 430074, China
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Received: 31 December 2015 / Revised: 31 January 2016 / Accepted: 4 February 2016 / Published: 10 February 2016
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Selenium is an essential micronutrient for humans. Much of selenium’s beneficial influence on health is attributed to its presence within 25 selenoproteins. Selenoprotein R (SelR), known as methionine sulfoxide reductase B1 (MsrB1), is a selenium-dependent enzyme that, like other Msrs, is required for lens cell viability. In order to investigate the roles of SelR in protecting human lens epithelial (hLE) cells against damage, the influences of SelR gene knockdown on D-galactose-induced apoptosis in hLE cells were studied. The results showed that both D-galactose and SelR gene knockdown by siRNA independently induced oxidative stress. When SelR-gene-silenced hLE cells were exposed to D-galactose, glucose-regulated protein 78 (GRP78) protein level was further increased, mitochondrial membrane potential was significantly decreased and accompanied by a release of mitochondrial cytochrome c. At the same time, the apoptosis cells percentage and the caspase-3 activity were visibly elevated in hLE cells. These results suggested that SelR might protect hLE cell mitochondria and mitigating apoptosis in hLE cells against oxidative stress and endoplasmic reticulum (ER) stress induced by D-galactose, implying that selenium as a micronutrient may play important roles in hLE cells. View Full-Text
Keywords: selenoprotein R; D-galactose; lens epithelial cells; apoptosis; oxidative stress; endoplasmic reticulum stress; mitochondrial membrane potential selenoprotein R; D-galactose; lens epithelial cells; apoptosis; oxidative stress; endoplasmic reticulum stress; mitochondrial membrane potential
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Dai, J.; Liu, H.; Zhou, J.; Huang, K. Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress. Int. J. Mol. Sci. 2016, 17, 231.

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