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Int. J. Mol. Sci. 2016, 17(12), 2053; doi:10.3390/ijms17122053

Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients

1
Institute of Pathology, Paracelsus Medical University/Salzburger Landeskliniken (SALK), Salzburg 5020, Austria
2
Laboratory for Tumour Biology and Experimental Therapies (TREAT), Institute of Physiology and Pathophysiology, Paracelsus Medical University, Salzburg 5020, Austria
3
Department of Internal Medicine I, Paracelsus Medical University/Salzburger Landeskliniken (SALK), Salzburg 5020, Austria
4
Department of Visceral-, Transplant- and Thoracic Surgery, Medical University of Innsbruck, Innsbruck 6020, Austria
5
Department of Surgery, Paracelsus Medical University/Salzburger Landeskliniken (SALK), Salzburg 5020, Austria
6
Research Unit for Non-Coding RNA and Genome Editing in Cancer, Division of Oncology, Department of Internal Medicine, Medical University of Graz, Graz 8036, Austria
7
Department for Radon Therapy Research, Ludwig Boltzmann Cluster for Arthritis and Rehabilitation, Salzburg 5020, Austria
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Constantinos Stathopoulos
Received: 3 November 2016 / Revised: 30 November 2016 / Accepted: 1 December 2016 / Published: 7 December 2016
(This article belongs to the Collection Regulation by Non-Coding RNAs)
View Full-Text   |   Download PDF [7866 KB, uploaded 7 December 2016]   |  

Abstract

Extensive stromal interaction is one reason for the dismal outcome of biliary tract cancer (BTC) patients. Epithelial to mesenchymal transition (EMT) is involved in tumor invasion and metastasis and is partly regulated by microRNAs (miRs). This study explores the expression of anti-EMT miR200 family (miR141, −200a/b/c, −429) and miR205 as well as the EMT-related proteins E-cadherin and vimentin in a panel of BTC cell lines and clinical specimens by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry, respectively. MicroRNA expression was correlated to (i) the expression patterns of E-cadherin and vimentin; (ii) clinicopathological characteristics; and (iii) survival data. MicroRNA-200 family and miR205 were expressed in all BTC cells and clinical specimens. E-cadherin and vimentin showed a mutually exclusive expression pattern in both, in vitro and in vivo. Expression of miR200 family members positively correlated with E-cadherin and negatively with vimentin expression in BTC cells and specimens. High expression of miR200 family members (but not miR205) and E-cadherin was associated with longer survival, while low miR200 family and high vimentin expression was a predictor of unfavorable survival. Overall, the current study demonstrates the relevance of the miR200 family in EMT of BTC tumors and suggests these miRs as predictors for positive outcome. View Full-Text
Keywords: biliary tract cancer; epithelial to mesenchymal transition; microRNA-200 family; microRNA-205 biliary tract cancer; epithelial to mesenchymal transition; microRNA-200 family; microRNA-205
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Urbas, R.; Mayr, C.; Klieser, E.; Fuereder, J.; Bach, D.; Stättner, S.; Primavesi, F.; Jaeger, T.; Stanzer, S.; Ress, A.L.; Löffelberger, M.; Wagner, A.; Berr, F.; Ritter, M.; Pichler, M.; Neureiter, D.; Kiesslich, T. Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients. Int. J. Mol. Sci. 2016, 17, 2053.

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