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Int. J. Mol. Sci. 2016, 17(12), 2052; doi:10.3390/ijms17122052

Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling

1
Eye Hospital, Nanjing Medical University, Nanjing 210000, China
2
Department of Ophthalmology, Wuxi No. 2 People’s Hospital, Nanjing Medical University, Wuxi 214002, China
3
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China
4
The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Claudio Bucolo and Chiara Maria Eandi
Received: 19 September 2016 / Revised: 24 November 2016 / Accepted: 30 November 2016 / Published: 7 December 2016
(This article belongs to the Special Issue Retinal Diseases: Bridging Basic and Clinical Research)
View Full-Text   |   Download PDF [4429 KB, uploaded 7 December 2016]   |  

Abstract

Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a complicated and serious type of rhegmatogenous retinal detachment (RRD). In this study, we identified differentially expressed proteins in the vitreous humors of RRDCD and RRD using isobaric tags for relative and absolute quantitation (iTRAQ) combined with nano-liquid chromatography-electrospray ion trap-mass spectrometry-mass spectrometry (nano-LC-ESI-MS/MS) and bioinformatic analysis. Our result shows that 103 differentially expressed proteins, including 54 up-regulated and 49 down-regulated proteins were identified in RRDCD. Gene ontology (GO) analysis suggested that most of the differentially expressed proteins were extracellular.The Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis suggested that proteins related to complement and coagulation cascades were significantly enriched. iTRAQ-based proteomic profiling reveals that complement and coagulation cascades and inflammation may play important roles in the pathogenesis of RRDCD. This study may provide novel insights into the pathogenesis of RRDCD and offer potential opportunities for the diagnosis and treatment of RRDCD. View Full-Text
Keywords: rhegmatogenous retinal detachment associated with choroidal detachment; rhegmatogenous retinal detachment; proteomics; iTRAQ rhegmatogenous retinal detachment associated with choroidal detachment; rhegmatogenous retinal detachment; proteomics; iTRAQ
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MDPI and ACS Style

Wu, Z.; Ding, N.; Yu, M.; Wang, K.; Luo, S.; Zou, W.; Zhou, Y.; Yan, B.; Jiang, Q. Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling. Int. J. Mol. Sci. 2016, 17, 2052.

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