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Int. J. Mol. Sci. 2016, 17(11), 1839; doi:10.3390/ijms17111839

Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines

1
Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil
2
Laboratory of Experimental Pathology, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil
3
Laboratory of Mutagenesis, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil
4
Medical School, Federal University of Minas Gerais—UFMG, Belo Horizonte 31270-901, Brazil
5
Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil
6
Laboratory of Tissue Processing, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil
7
Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga 4710-057, Portugal
8
3ICVS/3B’s-PT Government Associate Laboratory, Braga 4710-057, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Toshio Morikawa
Received: 1 September 2016 / Revised: 11 October 2016 / Accepted: 12 October 2016 / Published: 8 November 2016
(This article belongs to the Special Issue Biological Activity of Natural Secondary Metabolite Products)
View Full-Text   |   Download PDF [2582 KB, uploaded 8 November 2016]   |  

Abstract

Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy. View Full-Text
Keywords: HNSCC; cytotoxic activity; alkaloids; apoptosis markers HNSCC; cytotoxic activity; alkaloids; apoptosis markers
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MDPI and ACS Style

Silva-Oliveira, R.J.; Lopes, G.F.; Camargos, L.F.; Ribeiro, A.M.; Santos, F.V.; Severino, R.P.; Severino, V.G.P.; Terezan, A.P.; Thomé, R.G.; Santos, H.B.; Reis, R.M.; Ribeiro, R.I.M.A. Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines. Int. J. Mol. Sci. 2016, 17, 1839.

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