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Int. J. Mol. Sci. 2016, 17(11), 1815; doi:10.3390/ijms17111815

In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography

Molecular Imaging and Innovative Therapy in Oncology (IMOTION), EA 7435, University of Bordeaux, Bordeaux 33076, France
Author to whom correspondence should be addressed.
Academic Editor: Sundaresan Gobalakrishnan
Received: 29 August 2016 / Revised: 27 September 2016 / Accepted: 21 October 2016 / Published: 31 October 2016
(This article belongs to the Special Issue Cancer Molecular Imaging in the Era of Precision Medicine)
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Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuse optical tomography (fDOT), and fluorescence molecular Imaging (FMT®). A U87 cell line was genetically modified for constitutive expression of both the encoding Fluc and iRFP reporter genes and assayed for cell, subcutaneous tumor and brain tumor imaging. On cultured cells, BLI was more sensitive than FRI; in vivo, tumors were first detected by BLI. Fluorescence of iRFP provided convenient tools such as flux cytometry, direct detection of the fluorescent protein on histological slices, and fluorescent tomography that allowed for 3D localization and absolute quantification of the fluorescent signal in brain tumors. View Full-Text
Keywords: reporter gene; optical imaging; bioluminescence; fluorescence tomography; cancer; glioblastoma reporter gene; optical imaging; bioluminescence; fluorescence tomography; cancer; glioblastoma

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Genevois, C.; Loiseau, H.; Couillaud, F. In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography. Int. J. Mol. Sci. 2016, 17, 1815.

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