User | : | Dave |
---|---|---|
: | d.speijer@amc.uva.nl | |
MS data file | : | ext1861_E7_300_RT.mgf |
Database | : | SwissProt 2014_10 (546,790 sequences; 194,613,039 residues) |
Timestamp | : | 26 Nov 2014 at 14:44:45 GMT |
Not what you expected? Try the peptide summary.
Type of search | : | MS/MS Ion Search |
---|---|---|
Enzyme | : | Trypsin |
Fixed modifications | : | ![]() |
Variable modifications | : | ![]() |
Mass values | : | Monoisotopic |
Protein mass | : | Unrestricted |
Peptide mass tolerance | : | ± 0.3 Da |
Fragment mass tolerance | : | ± 0.4 Da |
Max missed cleavages | : | 1 |
Instrument type | : | ESI-TRAP |
Number of queries | : | 300 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.
There are 40 peptide matches above identity threshold and 50 matches above homology threshold for 300 queries. Histogram score range is (0, 105). On average, individual ions scores > 41 (beyond green shading) indicate identity or extensive homology (p<0.05).
[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
Modification | Site | Above thr. |
---|---|---|
Oxidation | M | 12 |
Carbamidomethyl | C | 2 |
Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
---|---|---|---|---|---|---|---|
0.037 | ![]() |
GAYSLSLR | significant | ||||
9 | ![]() |
GFFLFVEGGR | top ranking | ||||
6.4e-05 | ![]() |
GSSIFGLAPGK | significant and top ranking | ||||
1.3e-06 | ![]() |
![]() |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
6.2e-07 | ![]() |
![]() |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
6.4e-05 | ![]() |
U | GSSIFGLAPGK | unique | |||
![]() |
5.7e-05 | ![]() |
LNTLETEEWFFK | peptide has two duplicates | |||
0.18 | ![]() |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1
Accession | Score | Description | ||
---|---|---|---|---|
1 | TOP2A_HUMAN | 542 | DNA topoisomerase 2-alpha OS=Homo sapiens GN=TOP2A PE=1 SV=3 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | K1C10_HUMAN | 209 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | K2C1_HUMAN | 204 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | TRY1_BOVIN | 197 | Cationic trypsin OS=Bos taurus PE=1 SV=3 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | K1C9_HUMAN | 86 | Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | THIO_ECOLI | 71 | Thioredoxin-1 OS=Escherichia coli (strain K12) GN=trxA PE=1 SV=2 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | IGHG_RABIT | 37 | Ig gamma chain C region OS=Oryctolagus cuniculus PE=1 SV=1 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | EFTS_BREBN | 21 | Elongation factor Ts OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) GN=tsf PE=3 SV=1 |
1
Not what you expected? Try the peptide summary.