MASCOT Search Results

Search metadata
User : Dave
E-mail : d.speijer@amc.uva.nl
MS data file : ext1861_E7_300_RT.mgf
Database : SwissProt 2014_10 (546,790 sequences; 194,613,039 residues)
Timestamp : 26 Nov 2014 at 14:44:45 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.3 Da
Fragment mass tolerance : ± 0.4 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 300

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.

There are 40 peptide matches above identity threshold and 50 matches above homology threshold for 300 queries. Histogram score range is (0, 105). On average, individual ions scores > 41 (beyond green shading) indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Modification statistics
Modification Site Above thr.
Oxidation M 12
Carbamidomethyl C 2
Legend
Dupes Expect Rank U 1 2 Peptide
0.037 Expand2 GAYSLSLR significant
9 Expand1 GFFLFVEGGR top ranking
6.4e-05 Expand1 GSSIFGLAPGK significant and top ranking
1.3e-06 Expand1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-07 Expand1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-05 Expand1 U GSSIFGLAPGK unique
Expand2 5.7e-05 Expand1 LNTLETEEWFFK peptide has two duplicates
0.18 Expand1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (Expand) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (Collapse) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

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Taxonomy

Show

Protein families 1–8 (out of 8)


Page: 1 

Expand1

Accession Score Description
1 TOP2A_HUMAN 542 DNA topoisomerase 2-alpha OS=Homo sapiens GN=TOP2A PE=1 SV=3

Expand2

Accession Score Description
1 K1C10_HUMAN 209 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6

Expand3

Accession Score Description
1 K2C1_HUMAN 204 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

Expand4

Accession Score Description
1 TRY1_BOVIN 197 Cationic trypsin OS=Bos taurus PE=1 SV=3

Expand5

Accession Score Description
1 K1C9_HUMAN 86 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3

Expand6

Accession Score Description
1 THIO_ECOLI 71 Thioredoxin-1 OS=Escherichia coli (strain K12) GN=trxA PE=1 SV=2

Expand7

Accession Score Description
1 IGHG_RABIT 37 Ig gamma chain C region OS=Oryctolagus cuniculus PE=1 SV=1

Expand8

Accession Score Description
1 EFTS_BREBN 21 Elongation factor Ts OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) GN=tsf PE=3 SV=1

Page: 1 


Not what you expected? Try the peptide summary.