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Int. J. Mol. Sci. 2016, 17(11), 1756; doi:10.3390/ijms17111756

Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

1,2,3,†
,
1,2,3,†
,
4,†
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
5
,
5,* and 1,2,3,*
1
Key Laboratory of Biological and Medical Engineering, Guizhou Medical University, Guiyang 550025, China
2
Engineering Research Center of Medical Biotechnology, Guizhou Medical University, Guiyang 550025, China
3
School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, China
4
Department of Head and Neck, Affiliated Cancer Hospital, Guizhou Medical University, Guiyang 550025, China
5
Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing 100191, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Charles J. Malemud
Received: 6 July 2016 / Revised: 6 October 2016 / Accepted: 11 October 2016 / Published: 31 October 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [4156 KB, uploaded 31 October 2016]   |  

Abstract

Dendritic cells (DCs), the most potent antigen-presenting cells, play a central role in the initiation, regulation, and maintenance of the immune responses. Vascular endothelial growth factor (VEGF) is one of the important cytokines in the tumor microenvironment (TME) and can inhibit the differentiation and functional maturation of DCs. To elucidate the potential mechanisms of DC dysfunction induced by VEGF, the effects of VEGF on the biophysical characteristics and motility of human mature DCs (mDCs) were investigated. The results showed that VEGF had a negative influence on the biophysical properties, including electrophoretic mobility, osmotic fragility, viscoelasticity, and transmigration. Further cytoskeleton structure analysis by confocal microscope and gene expression profile analyses by gene microarray and real-time PCR indicated that the abnormal remodeling of F-actin cytoskeleton may be the main reason for the deterioration of biophysical properties, motility, and stimulatory capability of VEGF-treated mDCs. This is significant for understanding the biological behavior of DCs and the immune escape mechanism of tumors. Simultaneously, the therapeutic efficacies may be improved by blocking the signaling pathway of VEGF in an appropriate manner before the deployment of DC-based vaccinations against tumors. View Full-Text
Keywords: dendritic cells; vascular endothelial growth factor; biophysical characteristics; motility; immune function dendritic cells; vascular endothelial growth factor; biophysical characteristics; motility; immune function
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MDPI and ACS Style

Hu, Z.-Q.; Xue, H.; Long, J.-H.; Wang, Y.; Jia, Y.; Qiu, W.; Zhou, J.; Wen, Z.-Y.; Yao, W.-J.; Zeng, Z. Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling. Int. J. Mol. Sci. 2016, 17, 1756.

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