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Int. J. Mol. Sci. 2016, 17(11), 1124; doi:10.3390/ijms17111124

Upregulated MicroRNA-25 Mediates the Migration of Melanoma Cells by Targeting DKK3 through the WNT/β-Catenin Pathway

Department of Dermatology, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, China
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Author to whom correspondence should be addressed.
Academic Editor: Martin Pichler
Received: 14 April 2016 / Revised: 20 May 2016 / Accepted: 31 May 2016 / Published: 27 October 2016
(This article belongs to the Collection Regulation by Non-Coding RNAs)
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Abstract

Previous research indicates that microRNA-25 (miR-25) regulates carcinogenesis and the progression of various cancers, but the role of miR-25 in melanoma remains unclear. We observed that miR-25 was significantly upregulated in melanoma cell lines and tissue samples. Downregulation of miR-25 markedly suppressed invasion and proliferation of melanoma cells in vitro; however, overexpression of miR-25 markedly increased melanoma cell invasion and proliferation. Moreover, we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced β-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. In a nude xenograft model, miR-25 upregulation significantly increased A375 melanoma growth. In summary, miR-25 is upregulated in melanoma and promotes melanoma cell proliferation and invasion, partially by targeting DKK3. These results were indicated that miR-25 may serve as a potential target for the treatment of melanoma in the future. View Full-Text
Keywords: miR-25; DKK3; melanoma miR-25; DKK3; melanoma
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Huo, J.; Zhang, Y.; Li, R.; Wang, Y.; Wu, J.; Zhang, D. Upregulated MicroRNA-25 Mediates the Migration of Melanoma Cells by Targeting DKK3 through the WNT/β-Catenin Pathway. Int. J. Mol. Sci. 2016, 17, 1124.

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