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Int. J. Mol. Sci. 2016, 17(10), 1704; doi:10.3390/ijms17101704

In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editors: Christo Z. Christov and Tatyana Karabencheva-Christova
Received: 15 August 2016 / Revised: 26 September 2016 / Accepted: 29 September 2016 / Published: 11 October 2016
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
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Abstract

Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially in the studies of dynamic PPIs. To solve this problem, a method called Bioluminescence Resonance Energy Transfer (BRET) was developed about seventeen years ago. Since then, BRET has evolved into a whole class of methods that can be used to survey virtually any kinds of PPIs. Compared to many traditional methods, BRET is highly sensitive, reliable, easy to perform, and relatively inexpensive. However, most importantly, it can be done in vivo and allows the real-time monitoring of dynamic PPIs with the easily detectable light signal, which is extremely valuable for the PPI functional research. This review will take a comprehensive look at this powerful technique, including its principles, comparisons with other methods, experimental approaches, classifications, applications, early developments, recent progress, and prospects. View Full-Text
Keywords: protein–protein interactions; bioluminescence; fluorescence; Bioluminescence Resonance Energy Transfer; Förster Resonance Energy Transfer; NanoLuc; bacterial luciferase; protein dynamics protein–protein interactions; bioluminescence; fluorescence; Bioluminescence Resonance Energy Transfer; Förster Resonance Energy Transfer; NanoLuc; bacterial luciferase; protein dynamics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sun, S.; Yang, X.; Wang, Y.; Shen, X. In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. Int. J. Mol. Sci. 2016, 17, 1704.

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