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Int. J. Mol. Sci. 2016, 17(10), 1645; doi:10.3390/ijms17101645

Diatom Valve Three-Dimensional Representation: A New Imaging Method Based on Combined Microscopies

Institute for Microelectronics and Microsystems, Department of Naples, National Research Council, Naples 80131, Italy
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Academic Editor: Hermann Ehrlich
Received: 12 July 2016 / Revised: 30 August 2016 / Accepted: 23 September 2016 / Published: 28 September 2016
(This article belongs to the Special Issue Frontiers of Marine Biomaterials)
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Abstract

The frustule of diatoms, unicellular microalgae, shows very interesting photonic features, generally related to its complicated and quasi-periodic micro- and nano-structure. In order to simulate light propagation inside and through this natural structure, it is important to develop three-dimensional (3D) models for synthetic replica with high spatial resolution. In this paper, we present a new method that generates images of microscopic diatoms with high definition, by merging scanning electron microscopy and digital holography microscopy or atomic force microscopy data. Starting from two digital images, both acquired separately with standard characterization procedures, a high spatial resolution (Δz = λ/20, Δx = Δy ≅ 100 nm, at least) 3D model of the object has been generated. Then, the two sets of data have been processed by matrix formalism, using an original mathematical algorithm implemented on a commercially available software. The developed methodology could be also of broad interest in the design and fabrication of micro-opto-electro-mechanical systems. View Full-Text
Keywords: diatoms; three-dimensional image processing; digital holography; scanning microscopy diatoms; three-dimensional image processing; digital holography; scanning microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ferrara, M.A.; De Tommasi, E.; Coppola, G.; De Stefano, L.; Rea, I.; Dardano, P. Diatom Valve Three-Dimensional Representation: A New Imaging Method Based on Combined Microscopies. Int. J. Mol. Sci. 2016, 17, 1645.

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