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Int. J. Mol. Sci. 2016, 17(10), 1559; doi:10.3390/ijms17101559

Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites

1
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania
2
National Institute for Medical Research (NIMR), P.O. Box 9653, 14115 Dar es Salaam, Tanzania
*
Author to whom correspondence should be addressed.
Academic Editor: Már Másson
Received: 18 July 2016 / Revised: 23 August 2016 / Accepted: 30 August 2016 / Published: 27 September 2016
(This article belongs to the Special Issue Drug Delivery and Antimicrobial Agents)
View Full-Text   |   Download PDF [4298 KB, uploaded 27 September 2016]   |  

Abstract

Chitosan (CS, molecular weight 20.2 kDa, degree of deacylation (DD) 73.31%) was successfully obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the bioactive natural product, panchovillin (PANV), isolated from Erythrina schliebenii, on a chitosan-tripolyphosphate (CS/TPP) nano-framework was achieved by ionotropic gelation. Characterization of pure CS, CS/TPP and PANV-CS/TPP nanocomposites was performed by FTIR, SEM and XRD. The molecular weight of chitosan and the thermal stability of the materials were determined by MALDI-TOF-MS and simultaneous thermal analyzer (STA)/DTG, respectively. The respective encapsulation efficiency and loading capacity of the PANV were found to be 70% and 0.36%. The in vitro release studies showed an initial burst of 42% of PANV in the first six hours. This was followed by a slow and sustained release up to 72 h. The in vivo antimycobacterial activities of both PANV and PANV-CS/TPP nanocomposite against Mycobacterium indicus pranii (MIP) using Galleria mellonella larvae as an in vivo infection model are reported in this paper. View Full-Text
Keywords: panchovillin; chitosan; nanocomposites; antimycobacterial; Galleria mellonella panchovillin; chitosan; nanocomposites; antimycobacterial; Galleria mellonella
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MDPI and ACS Style

Rwegasila, E.; Mubofu, E.B.; Nyandoro, S.S.; Erasto, P.; Munissi, J.J.E. Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites. Int. J. Mol. Sci. 2016, 17, 1559.

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