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Int. J. Mol. Sci. 2015, 16(8), 18752-18777; doi:10.3390/ijms160818752

Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress

1
National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 12 June 2015 / Revised: 28 July 2015 / Accepted: 30 July 2015 / Published: 11 August 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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Abstract

Drought stress is one of the major abiotic factors affecting Brassica napus (B. napus) productivity. In order to identify genes of potential importance to drought stress and obtain a deeper understanding of the molecular mechanisms regarding the responses of B. napus to dehydration stress, we performed large-scale transcriptome sequencing of B. napus plants under dehydration stress using the Illumina sequencing technology. In this work, a relatively drought tolerant B. napus line, Q2, identified in our previous study, was used. Four cDNA libraries constructed from mRNAs of control and dehydration-treated root and leaf were sequenced by Illumina technology. A total of 6018 and 5377 differentially expressed genes (DEGs) were identified in root and leaf. In addition, 1745 genes exhibited a coordinated expression profile between the two tissues under drought stress, 1289 (approximately 74%) of which showed an inverse relationship, demonstrating different regulation patterns between the root and leaf. The gene ontology (GO) enrichment test indicated that up-regulated genes in root were mostly involved in “stimulus” “stress” biological process, and activated genes in leaf mainly functioned in “cell” “cell part” components. Furthermore, a comparative network related to plant hormone signal transduction and AREB/ABF, AP2/EREBP, NAC, WRKY and MYC/MYB transcription factors (TFs) provided a view of different stress tolerance mechanisms between root and leaf. Some of the DEGs identified may be candidates for future research aimed at detecting drought-responsive genes and will be useful for understanding the molecular mechanisms of drought tolerance in root and leaf of B. napus. View Full-Text
Keywords: Brassica napus; drought stress; root; leaf; transcriptome; differentially expressed gene Brassica napus; drought stress; root; leaf; transcriptome; differentially expressed gene
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, C.; Zhang, X.; Zhang, K.; An, H.; Hu, K.; Wen, J.; Shen, J.; Ma, C.; Yi, B.; Tu, J.; Fu, T. Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress. Int. J. Mol. Sci. 2015, 16, 18752-18777.

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