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Int. J. Mol. Sci. 2015, 16(8), 17826-17837; doi:10.3390/ijms160817826

Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults

1
Departments of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan
2
Center for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan
3
Department of Pharmacy, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan
4
School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
5
Department of Traditional Chinese Medicine, Chang Gung University, Linkow 244, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 12 June 2015 / Revised: 12 June 2015 / Accepted: 30 July 2015 / Published: 3 August 2015
(This article belongs to the Special Issue Nutrigenetics and Nutrigenomics)
View Full-Text   |   Download PDF [1033 KB, uploaded 3 August 2015]   |  

Abstract

Maternal nutrition can affect development, leading to long-term effects on the health of offspring. The most common outcome is programmed hypertension. We examined whether alterations in renal transcriptome are responsible for generating programmed hypertension among four different models using next-generation RNA sequencing (NGS) technology. Pregnant Sprague-Dawley rats received 50% caloric restriction (CR), intraperitoneal injection of 45 mg/kg streptozotocin, 60% high-fructose (HF) diet, or 1% NaCl in drinking water to conduct CR, diabetes, HF, or high-salt models, respectively. All four models induced programmed hypertension in adult male offspring. We observed 16 shared genes in a two-week-old kidney among four different models. The identified differential expressed genes (DEGs) that are related to the regulation of blood pressure included Adrb3, Alb, Apoe, Calca, Kng1, Adm2, Guca2b, Hba2, Hba-a2, and Ppara. The peroxisome proliferator-activated receptor (PPAR) signaling pathway and glutathione metabolism pathway were shared by the CR, diabetes, and HF models. Conclusively, a variety of maternal nutritional insults induced the same phenotype—programmed hypertension with differential alterations of renal transcriptome in adult male offspring. The roles of DEGs identified by the NGS in this study deserve further clarification to develop ideal maternal dietary interventions and thus spare the next generations from the burden of hypertension. View Full-Text
Keywords: developmental programming; diabetes; fructose; hypertension; next generation sequencing; nutrition developmental programming; diabetes; fructose; hypertension; next generation sequencing; nutrition
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tain, Y.-L.; Hsu, C.-N.; Chan, J.Y.H.; Huang, L.-T. Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults. Int. J. Mol. Sci. 2015, 16, 17826-17837.

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