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Int. J. Mol. Sci. 2015, 16(7), 16347-16377; doi:10.3390/ijms160716347

Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge

1
Fisheries College, Ocean University of China, Qingdao 266100, China
2
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 4 March 2015 / Revised: 26 June 2015 / Accepted: 29 June 2015 / Published: 17 July 2015
(This article belongs to the Special Issue Fish Molecular Biology)
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Abstract

Vibrio splendidus is identified as one of the major pathogenic factors for the skin ulceration syndrome in sea cucumber (Apostichopus japonicus), which has vastly limited the development of the sea cucumber culture industry. In order to screen the immune genes involving Vibrio splendidus challenge in sea cucumber and explore the molecular mechanism of this process, the related transcriptome and gene expression profiling of resistant and susceptible biotypes of sea cucumber with Vibrio splendidus challenge were collected for analysis. A total of 319,455,942 trimmed reads were obtained, which were assembled into 186,658 contigs. After that, 89,891 representative contigs (without isoform) were clustered. The analysis of the gene expression profiling identified 358 differentially expression genes (DEGs) in the bacterial-resistant group, and 102 DEGs in the bacterial-susceptible group, compared with that in control group. According to the reported references and annotation information from BLAST, GO and KEGG, 30 putative bacterial-resistant genes and 19 putative bacterial-susceptible genes were identified from DEGs. The qRT-PCR results were consistent with the RNA-Seq results. Furthermore, many DGEs were involved in immune signaling related pathways, such as Endocytosis, Lysosome, MAPK, Chemokine and the ERBB signaling pathway. View Full-Text
Keywords: sea cucumber (Apostichopus japonicus); Vibrio splendidus; transcriptome sequencing; differentially expressed genes; bacteria-resistant gene; bacteria-susceptible gene sea cucumber (Apostichopus japonicus); Vibrio splendidus; transcriptome sequencing; differentially expressed genes; bacteria-resistant gene; bacteria-susceptible gene
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gao, Q.; Liao, M.; Wang, Y.; Li, B.; Zhang, Z.; Rong, X.; Chen, G.; Wang, L. Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge. Int. J. Mol. Sci. 2015, 16, 16347-16377.

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