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Int. J. Mol. Sci. 2015, 16(7), 15609-15624; doi:10.3390/ijms160715609

Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation

1
IOM Ricerca, 95029 Viagrande, Italy
2
Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy
3
Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy
4
Department of Biomedical Sciences, Hematology Section, University of Catania, 95124 Catania, Italy
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Wenbin Deng
Received: 4 June 2015 / Revised: 24 June 2015 / Accepted: 4 July 2015 / Published: 9 July 2015
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Abstract

The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271 mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271 mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271 mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate. View Full-Text
Keywords: mesenchymal stromal cells; bone marrow; adipose tissue; adipogenic differentiation; chondrogenic differentiation; osteogenic differentiation; CD271 mesenchymal stromal cells; bone marrow; adipose tissue; adipogenic differentiation; chondrogenic differentiation; osteogenic differentiation; CD271
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Calabrese, G.; Giuffrida, R.; Lo Furno, D.; Parrinello, N.L.; Forte, S.; Gulino, R.; Colarossi, C.; Schinocca, L.R.; Giuffrida, R.; Cardile, V.; Memeo, L. Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation. Int. J. Mol. Sci. 2015, 16, 15609-15624.

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