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Int. J. Mol. Sci. 2015, 16(6), 14007-14038; doi:10.3390/ijms160614007

A Comparative Proteomic Analysis of the Buds and the Young Expanding Leaves of the Tea Plant (Camellia sinensis L.)

1,2,3,4,†
,
1,†
,
2
,
1,3,4
,
2,4
,
2
,
1
and
1,2,3,4,*
1
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China
2
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China
3
Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, Hunan, China
4
Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, Hunan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Setsuko Komatsu
Received: 15 April 2015 / Revised: 15 May 2015 / Accepted: 19 May 2015 / Published: 18 June 2015
(This article belongs to the Special Issue Plant Proteomic Research)
View Full-Text   |   Download PDF [2154 KB, uploaded 18 June 2015]   |  

Abstract

Tea (Camellia sinensis L.) is a perennial woody plant that is widely cultivated to produce a popular non-alcoholic beverage; this beverage has received much attention due to its pleasant flavor and bioactive ingredients, particularly several important secondary metabolites. Due to the significant changes in the metabolite contents of the buds and the young expanding leaves of tea plants, high-performance liquid chromatography (HPLC) analysis and isobaric tags for relative and absolute quantitation (iTRAQ) analysis were performed. A total of 233 differentially expressed proteins were identified. Among these, 116 proteins were up-regulated and 117 proteins were down-regulated in the young expanding leaves compared with the buds. A large array of diverse functions was revealed, including roles in energy and carbohydrate metabolism, secondary metabolite metabolism, nucleic acid and protein metabolism, and photosynthesis- and defense-related processes. These results suggest that polyphenol biosynthesis- and photosynthesis-related proteins regulate the secondary metabolite content of tea plants. The energy and antioxidant metabolism-related proteins may promote tea leaf development. However, reverse transcription quantitative real-time PCR (RT-qPCR) showed that the protein expression levels were not well correlated with the gene expression levels. These findings improve our understanding of the molecular mechanism of the changes in the metabolite content of the buds and the young expanding leaves of tea plants. View Full-Text
Keywords: Camellia sinensis L.; proteome; iTRAQ Camellia sinensis L.; proteome; iTRAQ
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, Q.; Li, J.; Liu, S.; Huang, J.; Lin, H.; Wang, K.; Cheng, X.; Liu, Z. A Comparative Proteomic Analysis of the Buds and the Young Expanding Leaves of the Tea Plant (Camellia sinensis L.). Int. J. Mol. Sci. 2015, 16, 14007-14038.

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