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Int. J. Mol. Sci. 2015, 16(6), 13692-13713; doi:10.3390/ijms160613692

Proteomic Analysis of Immature Fraxinus mandshurica Cotyledon Tissues during Somatic Embryogenesis: Effects of Explant Browning on Somatic Embryogenesis

State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Hexing Road 26, Harbin 150040, China
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Academic Editor: David Sheehan
Received: 26 March 2015 / Accepted: 10 June 2015 / Published: 15 June 2015
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Manchurian ash (Fraxinus mandshurica Rupr.) is a valuable hardwood species in Northeast China. In cultures of F. mandshurica, somatic embryos were produced mainly on browned explants. Therefore, we studied the mechanism of explant browning and its relationship with somatic embryogenesis (SE). We used explants derived from F. mandshurica immature zygotic embryo cotyledons as materials. Proteins were extracted from browned embryogenic explants, browned non-embryogenic explants, and non-brown explants, and then separated by 2-dimensional electrophoresis. Differentially and specifically expressed proteins were analyzed by mass spectrometry to identify proteins involved in the browning of explants and SE. Some stress response and defense proteins such as chitinases, peroxidases, aspartic proteinases, and an osmotin-like protein played important roles during SE of F. mandshurica. Our results indicated that explant browning might not be caused by the accumulation and oxidation of polyphenols only, but also by some stress-related processes, which were involved in programmed cell death (PCD), and then induced SE. View Full-Text
Keywords: manchurian ash; mass spectrometry; PCD; somatic embryos; stress response manchurian ash; mass spectrometry; PCD; somatic embryos; stress response
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Liu, C.-P.; Yang, L.; Shen, H.-L. Proteomic Analysis of Immature Fraxinus mandshurica Cotyledon Tissues during Somatic Embryogenesis: Effects of Explant Browning on Somatic Embryogenesis. Int. J. Mol. Sci. 2015, 16, 13692-13713.

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