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Open AccessCommunication
Int. J. Mol. Sci. 2015, 16(6), 13653-13660; doi:10.3390/ijms160613653

Enzymatic Polymerization on DNA Modified Gold Nanowire for Label-Free Detection of Pathogen DNA

Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea
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Author to whom correspondence should be addressed.
Academic Editor: Vladimír Křen
Received: 11 May 2015 / Revised: 8 June 2015 / Accepted: 10 June 2015 / Published: 15 June 2015
(This article belongs to the Special Issue Molecular Biocatalysis)
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Abstract

This paper presents a label-free biosensor for the detection of single-stranded pathogen DNA through the target-enhanced gelation between gold nanowires (AuNW) and the primer DNAs branched on AuNW. The target DNA enables circularization of the linear DNA template, and the primer DNA is elongated continuously via rolling circle amplification. As a result, in the presence of the target DNA, a macroscopic hydrogel was fabricated by the entanglement of the elongated DNA with AuNWs as a scaffold fiber for effective gelation. In contrast, very small separate particles were generated in the absence of the target DNA. This label-free biosensor might be a promising tool for the detection of pathogen DNAs without any devices for further analysis. Moreover, the biosensor based on the weaving of AuNW and DNAs suggests a novel direction for the applications of AuNWs in biological engineering. View Full-Text
Keywords: biosensor; label-free; pathogen DNA detection; rolling circle amplification (RCA); gold nanowire biosensor; label-free; pathogen DNA detection; rolling circle amplification (RCA); gold nanowire
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jeong, J.; Kim, H.; Lee, J.B. Enzymatic Polymerization on DNA Modified Gold Nanowire for Label-Free Detection of Pathogen DNA. Int. J. Mol. Sci. 2015, 16, 13653-13660.

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