Next Article in Journal
A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family
Next Article in Special Issue
Comparison of Selective Laser Melted Titanium and Magnesium Implants Coated with PCL
Previous Article in Journal
From Pre-Clinical Studies to Clinical Trials: Generation of Novel Therapies for Pregnancy Complications
Previous Article in Special Issue
Encapsulated Cellular Implants for Recombinant Protein Delivery and Therapeutic Modulation of the Immune System
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(6), 12925-12942; doi:10.3390/ijms160612925

The Effect of Electrospun Gelatin Fibers Alignment on Schwann Cell and Axon Behavior and Organization in the Perspective of Artificial Nerve Design

1
Department of Clinical and Biological Sciences, University of Torino, Orbassano 10043, Italy
2
Neuroscience Institute of the Cavalieri-Ottolenghi Foundation, University of Torino, Orbassano 10043, Italy
3
Department of Mechanical and Aerospace Engineering, Politecnico of Torino, Torino 10100, Italy
4
Nanostructured Interfaces and Surfaces, Department of Chemistry, University of Torino, Torino 10100, Italy
5
Department for Materials and Devices of the National Research Council, Institute for the Cehmical and Physical Processes (CNR-IPCF UOS), Pisa 56124, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Aaron Tan
Received: 31 March 2015 / Revised: 26 May 2015 / Accepted: 29 May 2015 / Published: 8 June 2015
(This article belongs to the Special Issue Artificial Organs)
View Full-Text   |   Download PDF [4679 KB, uploaded 8 June 2015]   |  

Abstract

Electrospun fibrous substrates mimicking extracellular matrices can be prepared by electrospinning, yielding aligned fibrous matrices as internal fillers to manufacture artificial nerves. Gelatin aligned nano-fibers were prepared by electrospinning after tuning the collector rotation speed. The effect of alignment on cell adhesion and proliferation was tested in vitro using primary cultures, the Schwann cell line, RT4-D6P2T, and the sensory neuron-like cell line, 50B11. Cell adhesion and proliferation were assessed by quantifying at several time-points. Aligned nano-fibers reduced adhesion and proliferation rate compared with random fibers. Schwann cell morphology and organization were investigated by immunostaining of the cytoskeleton. Cells were elongated with their longitudinal body parallel to the aligned fibers. B5011 neuron-like cells were aligned and had parallel axon growth when cultured on the aligned gelatin fibers. The data show that the alignment of electrospun gelatin fibers can modulate Schwann cells and axon organization in vitro, suggesting that this substrate shows promise as an internal filler for the design of artificial nerves for peripheral nerve reconstruction. View Full-Text
Keywords: peripheral nerve injury; artificial nerve organs; gelatin nano-fibers; electrospinning; aligned fibers peripheral nerve injury; artificial nerve organs; gelatin nano-fibers; electrospinning; aligned fibers
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gnavi, S.; Fornasari, B.E.; Tonda-Turo, C.; Laurano, R.; Zanetti, M.; Ciardelli, G.; Geuna, S. The Effect of Electrospun Gelatin Fibers Alignment on Schwann Cell and Axon Behavior and Organization in the Perspective of Artificial Nerve Design. Int. J. Mol. Sci. 2015, 16, 12925-12942.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top