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Int. J. Mol. Sci. 2015, 16(6), 12360-12367; doi:10.3390/ijms160612360

Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues

Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Viale delle Scienze, Universita' degli Studi di Palermo, Palermo 90128, Italy
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Kotb Abdelmohsen
Received: 10 April 2015 / Revised: 24 May 2015 / Accepted: 25 May 2015 / Published: 1 June 2015
(This article belongs to the Special Issue Post-Transcriptional Gene Regulation by Ribonucleoprotein Complexes)
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Abstract

The identification and study of protein’s function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila’s embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila’s larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation. View Full-Text
Keywords: Drosophila; hnRNPs; proteins extraction Drosophila; hnRNPs; proteins extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Piccolo, L.L.; Bonaccorso, R.; Onorati, M.C. Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues. Int. J. Mol. Sci. 2015, 16, 12360-12367.

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