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Int. J. Mol. Sci. 2015, 16(5), 9217-9235; doi:10.3390/ijms16059217

Cellular Anti-Melanogenic Effects of a Euryale ferox Seed Extract Ethyl Acetate Fraction via the Lysosomal Degradation Machinery

1
Department of Food Science & Biotechnology, Graduate School, Kyungpook National University, Daegu 702-701, Korea
2
Department of Nano-Science & Technology, Graduate School, Kyungpook National University, Daegu 702-701, Korea
3
Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Manickam Sugumaran
Received: 5 December 2014 / Revised: 17 April 2015 / Accepted: 20 April 2015 / Published: 23 April 2015
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Abstract

The aim of this study was to investigate the effect of ethyl acetate fraction of Euryale ferox seed extracts (Efse-EA) on melanogenesis in immortalized mouse melanocyte cell line, melan-a. Efse-EA showed strong dose-dependent mushroom tyrosinase inhibitory activity. Treatment of melan-a cells with 30 μg/mL Efse-EA produced strong inhibition of cellular tyrosinase and melanin synthesis. Efse-EA significantly reduced the levels of melanogenesis-related proteins, such as tyrosinase, tyrosinase-related proteins 1 and 2, and microphthalmia-associated transcription factor. Because Efse-EA treatment reduced tyrosinase protein levels without changing its mRNA expression, we investigated whether this decrease was related to proteasomal or lysosomal degradation of tyrosinase. We found that chloroquine, a lysosomal proteolysis inhibitor, almost completely abolished both the down-regulation of tyrosinase and the inhibition of melanin synthesis induced by Efse-EA. These results suggested that Efse-EA may contribute to the inhibition of melanogenesis by altering lysosomal degradation of tyrosinase, and that this extract may provide a new cosmetic skin-whitening agent. View Full-Text
Keywords: Euryale ferox; seed; melanin; tyrosinase; lysosomal degradation Euryale ferox; seed; melanin; tyrosinase; lysosomal degradation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Baek, S.-H.; Nam, I.-J.; Kwak, H.S.; Kim, K.-C.; Lee, S.-H. Cellular Anti-Melanogenic Effects of a Euryale ferox Seed Extract Ethyl Acetate Fraction via the Lysosomal Degradation Machinery. Int. J. Mol. Sci. 2015, 16, 9217-9235.

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