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Int. J. Mol. Sci. 2015, 16(5), 9037-9063; doi:10.3390/ijms16059037

Overexpressing of OsAMT1-3, a High Affinity Ammonium Transporter Gene, Modifies Rice Growth and Carbon-Nitrogen Metabolic Status

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Microelement Research Center, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
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Academic Editor: Lam-Son Phan Tran
Received: 15 March 2015 / Revised: 11 April 2015 / Accepted: 13 April 2015 / Published: 23 April 2015
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Abstract

AMT1-3 encodes the high affinity NH4+ transporter in rice roots and is predominantly expressed under nitrogen starvation. In order to evaluate the effect of AMT1-3 gene on rice growth, nitrogen absorption and metabolism, we generated AMT1-3-overexpressing plants and analyzed the growth phenotype, yield, carbon and nitrogen metabolic status, and gene expression profiles. Although AMT1-3 mRNA accumulated in transgenic plants, these plants displayed significant decreases in growth when compared to the wild-type plants. The nitrogen uptake assay using a 15N tracer revealed poor nitrogen uptake ability in AMT1-3-overexpressing plants. We found significant decreases in AMT1-3-overexpressing plant leaf carbon and nitrogen content accompanied with a higher leaf C/N ratio. Significant changes in soluble proteins and carbohydrates were also observed in AMT1-3-overexpressing plants. In addition, metabolite profile analysis demonstrated significant changes in individual sugars, organic acids and free amino acids. Gene expression analysis revealed distinct expression patterns of genes that participate in carbon and nitrogen metabolism. Additionally, the correlation between the metabolites and gene expression patterns was consistent in AMT1-3-overexpressing plants under both low and high nitrogen growth conditions. Therefore, we hypothesized that the carbon and nitrogen metabolic imbalance caused by AMT1-3 overexpressing attributed to the poor growth and yield of transgenic plants. View Full-Text
Keywords: AMT1-3; carbon-nitrogen metabolism; 15N tracer; overexpressing; rice AMT1-3; carbon-nitrogen metabolism; 15N tracer; overexpressing; rice
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Bao, A.; Liang, Z.; Zhao, Z.; Cai, H. Overexpressing of OsAMT1-3, a High Affinity Ammonium Transporter Gene, Modifies Rice Growth and Carbon-Nitrogen Metabolic Status. Int. J. Mol. Sci. 2015, 16, 9037-9063.

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