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Int. J. Mol. Sci. 2015, 16(4), 6890-6901; doi:10.3390/ijms16046890

Tumour Cell Membrane Poration and Ablation by Pulsed Low-Intensity Electric Field with Carbon Nanotubes

Institute for Medical Science and Technology (IMSaT), College of Medicine, Dentistry and Nursing, University of Dundee, Dundee DD2 1FD, UK
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 3 February 2015 / Revised: 19 March 2015 / Accepted: 23 March 2015 / Published: 26 March 2015
(This article belongs to the Collection Bioactive Nanoparticles)
View Full-Text   |   Download PDF [1393 KB, uploaded 26 March 2015]   |  

Abstract

Electroporation is a physical method to increase permeabilization of cell membrane by electrical pulses. Carbon nanotubes (CNTs) can potentially act like “lighting rods” or exhibit direct physical force on cell membrane under alternating electromagnetic fields thus reducing the required field strength. A cell poration/ablation system was built for exploring these effects of CNTs in which two-electrode sets were constructed and two perpendicular electric fields could be generated sequentially. By applying this system to breast cancer cells in the presence of multi-walled CNTs (MWCNTs), the effective pulse amplitude was reduced to 50 V/cm (main field)/15 V/cm (alignment field) at the optimized pulse frequency (5 Hz) of 500 pulses. Under these conditions instant cell membrane permeabilization was increased to 38.62%, 2.77-fold higher than that without CNTs. Moreover, we also observed irreversible electroporation occurred under these conditions, such that only 39.23% of the cells were viable 24 h post treatment, in contrast to 87.01% cell viability without presence of CNTs. These results indicate that CNT-enhanced electroporation has the potential for tumour cell ablation by significantly lower electric fields than that in conventional electroporation therapy thus avoiding potential risks associated with the use of high intensity electric pulses. View Full-Text
Keywords: electrical pulse; electroporation; tumour cell ablation; carbon nanotubes; cell membrane permeabilization electrical pulse; electroporation; tumour cell ablation; carbon nanotubes; cell membrane permeabilization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, L.; Liu, D.; Zhou, R.; Wang, Z.; Cuschieri, A. Tumour Cell Membrane Poration and Ablation by Pulsed Low-Intensity Electric Field with Carbon Nanotubes. Int. J. Mol. Sci. 2015, 16, 6890-6901.

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