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Int. J. Mol. Sci. 2015, 16(3), 6513-6531; doi:10.3390/ijms16036513

Recent Developments of Engineered Translational Machineries for the Incorporation of Non-Canonical Amino Acids into Polypeptides

1
Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
2
Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Ibba
Received: 14 January 2015 / Revised: 13 March 2015 / Accepted: 16 March 2015 / Published: 20 March 2015
(This article belongs to the Special Issue Functions of Transfer RNAs)
View Full-Text   |   Download PDF [2092 KB, uploaded 20 March 2015]   |  

Abstract

Genetic code expansion and reprogramming methodologies allow us to incorporate non-canonical amino acids (ncAAs) bearing various functional groups, such as fluorescent groups, bioorthogonal functional groups, and post-translational modifications, into a desired position or multiple positions in polypeptides both in vitro and in vivo. In order to efficiently incorporate a wide range of ncAAs, several methodologies have been developed, such as orthogonal aminoacyl-tRNA-synthetase (AARS)–tRNA pairs, aminoacylation ribozymes, frame-shift suppression of quadruplet codons, and engineered ribosomes. More recently, it has been reported that an engineered translation system specifically utilizes an artificially built genetic code and functions orthogonally to naturally occurring counterpart. In this review we summarize recent advances in the field of ribosomal polypeptide synthesis containing ncAAs. View Full-Text
Keywords: tRNA; ribosome; non-canonical amino acids (ncAAs); aminoacyl-tRNA synthetase (AARS); ribozyme; genetic code expansion; genetic code reprogramming tRNA; ribosome; non-canonical amino acids (ncAAs); aminoacyl-tRNA synthetase (AARS); ribozyme; genetic code expansion; genetic code reprogramming
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Terasaka, N.; Iwane, Y.; Geiermann, A.-S.; Goto, Y.; Suga, H. Recent Developments of Engineered Translational Machineries for the Incorporation of Non-Canonical Amino Acids into Polypeptides. Int. J. Mol. Sci. 2015, 16, 6513-6531.

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