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Int. J. Mol. Sci. 2015, 16(3), 5803-5829; doi:10.3390/ijms16035803

Methodologies and Perspectives of Proteomics Applied to Filamentous Fungi: From Sample Preparation to Secretome Analysis

UTTRI-GENER Genetics and Genomics for Energy and Environment Laboratory—ENEA TRISAIA Research Center, 75025 Rotondella (Matera), Italy
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Academic Editor: David Sheehan
Received: 19 December 2014 / Revised: 17 February 2015 / Accepted: 3 March 2015 / Published: 12 March 2015
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Filamentous fungi possess the extraordinary ability to digest complex biomasses and mineralize numerous xenobiotics, as consequence of their aptitude to sensing the environment and regulating their intra and extra cellular proteins, producing drastic changes in proteome and secretome composition. Recent advancement in proteomic technologies offers an exciting opportunity to reveal the fluctuations of fungal proteins and enzymes, responsible for their metabolic adaptation to a large variety of environmental conditions. Here, an overview of the most commonly used proteomic strategies will be provided; this paper will range from sample preparation to gel-free and gel-based proteomics, discussing pros and cons of each mentioned state-of-the-art technique. The main focus will be kept on filamentous fungi. Due to the biotechnological relevance of lignocellulose degrading fungi, special attention will be finally given to their extracellular proteome, or secretome. Secreted proteins and enzymes will be discussed in relation to their involvement in bio-based processes, such as biomass deconstruction and mycoremediation. View Full-Text
Keywords: fungal proteomics; secretome; filamentous fungi; lignocellulose bioconversion; fungal degradation fungal proteomics; secretome; filamentous fungi; lignocellulose bioconversion; fungal degradation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bianco, L.; Perrotta, G. Methodologies and Perspectives of Proteomics Applied to Filamentous Fungi: From Sample Preparation to Secretome Analysis. Int. J. Mol. Sci. 2015, 16, 5803-5829.

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