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Int. J. Mol. Sci. 2015, 16(3), 5434-5451; doi:10.3390/ijms16035434

SOCS3 Silencing Attenuates Eosinophil Functions in Asthma Patients

1
Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain
2
Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain
3
Department of Allergy, Fundación Jiménez Díaz, 28040 Madrid, Spain
4
Department of Allergy, Hospital La Paz Health Research Institute (IdiPAZ), 28046 Madrid, Spain
5
Department of Allergy, Hospital Infanta Elena, Valdemoro, 28342 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 21 January 2015 / Revised: 25 February 2015 / Accepted: 5 March 2015 / Published: 10 March 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process. View Full-Text
Keywords: short interfering RNAs (siRNAs); SOCS3; eosinophils; asthma short interfering RNAs (siRNAs); SOCS3; eosinophils; asthma
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zafra, M.P.; Cañas, J.A.; Mazzeo, C.; Gámez, C.; Sanz, V.; Fernández-Nieto, M.; Quirce, S.; Barranco, P.; Ruiz-Hornillos, J.; Sastre, J.; del Pozo, V. SOCS3 Silencing Attenuates Eosinophil Functions in Asthma Patients. Int. J. Mol. Sci. 2015, 16, 5434-5451.

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