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Int. J. Mol. Sci. 2015, 16(2), 2767-2793; doi:10.3390/ijms16022767

Molecular Characterization of Three Gonadotropin Subunits and Their Expression Patterns during Ovarian Maturation in Cynoglossus semilaevis

Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China
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Academic Editor: Helmut Segner
Received: 21 November 2014 / Revised: 19 December 2014 / Accepted: 16 January 2015 / Published: 27 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The endocrine regulation of reproduction in a multiple spawning flatfish with an ovary of asynchronous development remains largely unknown. The objectives of this study were to monitor changes in mRNA expression patterns of three gonadotropin hormone (GTH) subunits (FSHβ, LHβ and CGα) and plasma GTH levels during ovarian maturation of half-smooth tongue sole Cynoglossus semilaevis. Cloning and sequence analysis revealed that the cDNAs of FSHβ, LHβ and CGα were 541, 670 and 685 bp in length, and encode for peptides of 130, 158 and 127 amino acids, respectively. The number of cysteine residues and potential N-linked glycosylation sites of the flatfish GTHs were conserved among teleosts. However, the primary structure of GTHs in Pleuronectiformes appeared to be highly divergent. The FSHβ transcriptional level in the pituitary remained high during the vitellogenic stage while plasma levels of FSH peaked and oocyte development was stimulated. The LHβ expression in the pituitary and ovary reached the maximum level during oocyte maturation stages when the plasma levels of LH peaked. The brain GTHs were expressed at the different ovarian stages. These results suggested that FSH and LH may simultaneously regulate ovarian development and maturation through the brain-pituitary-ovary axis endocrine system in tongue sole. View Full-Text
Keywords: Cynoglossus semilaevis; multiple spawner; gonadotropin; cDNA cloning; expression patterns; oocyte maturation Cynoglossus semilaevis; multiple spawner; gonadotropin; cDNA cloning; expression patterns; oocyte maturation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shi, B.; Liu, X.; Xu, Y.; Wang, S. Molecular Characterization of Three Gonadotropin Subunits and Their Expression Patterns during Ovarian Maturation in Cynoglossus semilaevis. Int. J. Mol. Sci. 2015, 16, 2767-2793.

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