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Int. J. Mol. Sci. 2015, 16(12), 29900-29910; doi:10.3390/ijms161226211

Effect of Guibi-Tang, a Traditional Herbal Formula, on Retinal Neovascularization in a Mouse Model of Proliferative Retinopathy

KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
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Academic Editor: Joseph V. Moxon
Received: 30 October 2015 / Revised: 9 December 2015 / Accepted: 9 December 2015 / Published: 16 December 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Ocular pathologic angiogenesis is an important causative risk factor of blindness in retinopathy of prematurity, proliferative diabetic retinopathy, and neovascular macular degeneration. Guibi-tang (GBT) is a frequently used oriental herbal formula in East Asian countries, and is also called Qui-pi-tang in Chinese and Kihi-To in Japanese. In the present study, we investigated the preventive effect of GBT on retinal pathogenic neovascularization in a mouse model of oxygen-induced retinopathy (OIR). C57BL/6 mice were exposed to 75% hyperoxia for five days on postnatal day 7 (P7). The mice were then exposed to room air from P12 to P17 to induce ischemic proliferative retinopathy. GBT (50 or 100 mg/kg/day) was intraperitoneally administered daily for five days (from P12 to P16). On P17, Retinal neovascularization was measured on P17, and the expression levels of 55 angiogenesis-related factors were analyzed using protein arrays. GBT significantly decreased retinal pathogenic angiogenesis in OIR mice, and protein arrays revealed that GBT decreased PAI-1 protein expression levels. Quantitative real-time PCR revealed that GBT reduced vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF2), and plasminogen activator inhibitor 1 (PAI-1) mRNA levels in OIR mice. GBT promotes potent inhibitory activity for retinal neovascularization by decreasing VEGF, FGF2, and PAI-1 levels. View Full-Text
Keywords: retinal neovascularization; fibroblast growth factor 2; plasminogen activator inhibitor 1; vascular endothelial growth factor; oxygen-induced retinopathy retinal neovascularization; fibroblast growth factor 2; plasminogen activator inhibitor 1; vascular endothelial growth factor; oxygen-induced retinopathy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lee, Y.M.; Lee, Y.-R.; Kim, C.-S.; Jo, K.; Sohn, E.; Kim, J.S.; Kim, J. Effect of Guibi-Tang, a Traditional Herbal Formula, on Retinal Neovascularization in a Mouse Model of Proliferative Retinopathy. Int. J. Mol. Sci. 2015, 16, 29900-29910.

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