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Int. J. Mol. Sci. 2015, 16(12), 29663-29672; doi:10.3390/ijms161226193

Comparative Studies of 5S rDNA Profiles and Cyt b Sequences in two Onychostoma Species (Cyprinidae)

National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan
Graduate Institute of Marine Biology, National Dong Hwa University, Hualien 974, Taiwan
Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
Institute of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 24 September 2015 / Revised: 27 November 2015 / Accepted: 1 December 2015 / Published: 11 December 2015
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [1951 KB, uploaded 11 December 2015]   |  


Onychostoma barbatulum and O. alticorpus, two primarily freshwater cyprinid fish, have similar morphological characters and partially overlapping ecological habitats. In order to explore the genetic differences between these two species, chromosomal characteristics and genetic variations were examined by fluorescence in situ hybridization (FISH) of 5S rDNA and cytochrome (Cyt) b gene analysis. Ten specimens of O. barbatulum and O. alticorpus were collected from the Nanzihsian Stream in southern Taiwan. FISH revealed that the 5S rDNA loci of O. barbatulum and O. alticorpus were found at a pericentromeric and subtelomeric position, respectively, in a pair of submetacentric chromosomes. Cyt b genes were amplified and sequenced from five individuals of each species. Intraspecific genetic distances ranged from 0.001–0.004 in O. barbatulum and from 0.001–0.006 in O. alticorpus. Genetic distances between these two species ranged from 0.132–0.142. The phylogenetic tree showed these two species are not sister species. In conclusion, FISH cytogenetic information and Cyt b gene analyses indicated that these two species have significantly different genetic characteristics; nevertheless, their morphological similarities may be due to environmental adaptation. View Full-Text
Keywords: adaptation; cyprinid; cytogenetic; genetic distance; Taiwan adaptation; cyprinid; cytogenetic; genetic distance; Taiwan

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Han, C.-C.; Yen, T.-B.; Chen, N.-C.; Tseng, M.-C. Comparative Studies of 5S rDNA Profiles and Cyt b Sequences in two Onychostoma Species (Cyprinidae). Int. J. Mol. Sci. 2015, 16, 29663-29672.

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