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Int. J. Mol. Sci. 2015, 16(12), 29496-29507; doi:10.3390/ijms161226179

Calycosin Suppresses RANKL-Mediated Osteoclastogenesis through Inhibition of MAPKs and NF-κB

1
Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, Jilin University Bethune Second Hospital, Changchun 130041, China
2
School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
3
Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China
4
Department of Virus Disease Prevention and Control, Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Gopinadhan Paliyath and Sanjay K. Srivastava
Received: 27 October 2015 / Revised: 2 December 2015 / Accepted: 2 December 2015 / Published: 10 December 2015
(This article belongs to the Special Issue The Mechanism of Action of Food Components in Disease Prevention)
View Full-Text   |   Download PDF [3707 KB, uploaded 10 December 2015]   |  

Abstract

Calycosin, an isoflavonoid phytoestrogen, isolated from Radix Astragali, was reported to possess anti-tumor, anti-inflammation, and osteogenic properties, but its impact on osteoclast differentiation remains unclear. In this study, we examined the effects of calycosin on osteoclastogenesis induced by RANKL. The results showed that calycosin significantly inhibited RANKL-induced osteoclast formation from primary bone marrow macrophages (BMMs). Calycosin also dose-dependently suppressed the formation of bone resorption pits by mature osteoclasts. In addition, the expression of osteoclatogenesis-related genes, including cathepsin K (CtsK), tartrate-resistant acid phosphatase (TRAP), and MMP-9, was significantly inhibited by calycosin. Furthermore, the results indicated that calycosin down-regulated the expression levels of NFATc1 and c-Fos through suppressing the activation of NF-κB and MAPKs. Our results indicate that calycosin has an inhibitory role in the bone loss by preventing osteoclast formation, as well as its bone resorptive activity. Therefore, calycosin may be useful as a therapeutic reagent for bone loss-associated diseases. View Full-Text
Keywords: calycosin; osteoclast; bone resorption; NFATc1; c-Fos; NF-κB; MAPKs calycosin; osteoclast; bone resorption; NFATc1; c-Fos; NF-κB; MAPKs
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Quan, G.-H.; Wang, H.; Cao, J.; Zhang, Y.; Wu, D.; Peng, Q.; Liu, N.; Sun, W.-C. Calycosin Suppresses RANKL-Mediated Osteoclastogenesis through Inhibition of MAPKs and NF-κB. Int. J. Mol. Sci. 2015, 16, 29496-29507.

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