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Int. J. Mol. Sci. 2015, 16(12), 29467-29481; doi:10.3390/ijms161226186

Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor

1
Department of Physiology, Shantou University Medical College, Shantou 515041, China
2
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China
3
Department of Surgery, Guangzhou Medical University, Guangzhou 510182, China
4
Department of Surgery, Anhui Medical University, Hefei 230032, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alan C. Leonard
Received: 14 August 2015 / Revised: 26 November 2015 / Accepted: 2 December 2015 / Published: 10 December 2015
(This article belongs to the Special Issue Molecular Machinery of Cell Growth Regulation)
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Abstract

Androgens and the androgen receptor (AR) are of great importance to spermatogenesis and male fertility. AR knockout (ARKO) mice display a complete insensitivity to androgens and male infertility; however, the exact molecular mechanism for this effect remains unclear. In this study, we found that the expression levels of Prmt6 mRNA and protein were significantly up-regulated in the testes of ARKO mice compared to wild type (WT) mice. PRMT6 was principally localized to the nucleus of spermatogonia and spermatocytes by immunofluorescence staining. Furthermore, luciferase assay data showed that AR together with testosterone treatment suppressed Prmt6 transcription via binding to the androgen-responsive element (ARE) of the Prmt6 promoter. Moreover, knockdown of Prmt6 suppressed germ cells migration and promoted apoptosis. In addition, both of these cellular activities could not be enhanced by testosterone treatment. Taken together, these data indicate that PRMT6, which was down-regulated by AR and influenced cell migration and apoptosis of germ cells, could play a potentially important role in spermatogenesis. View Full-Text
Keywords: PRMT6; androgen receptor; testes; migration; apoptosis PRMT6; androgen receptor; testes; migration; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Luo, M.; Li, Y.; Guo, H.; Lin, S.; Chen, J.; Ma, Q.; Gu, Y.; Jiang, Z.; Gui, Y. Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor. Int. J. Mol. Sci. 2015, 16, 29467-29481.

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