Next Article in Journal
ITSN2L Interacts with and Negatively Regulates RABEP1
Next Article in Special Issue
Cell Penetrating Peptide Conjugated Chitosan for Enhanced Delivery of Nucleic Acid
Previous Article in Journal
Therapeutic Targets for Neurodevelopmental Disorders Emerging from Animal Models with Perinatal Immune Activation
Previous Article in Special Issue
Biodistribution, Stability, and Blood Distribution of the Cell Penetrating Peptide Maurocalcine in Mice
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2015, 16(12), 28230-28241; doi:10.3390/ijms161226094

Therapeutic Potential of Cell Penetrating Peptides (CPPs) and Cationic Polymers for Chronic Hepatitis B

1
Institut National de la Sante et Recherche Medicale (INSERM) U1052, Cancer Research Center of Lyon (CRCL), Lyon 69003, France
2
Département de Biologie Cellulaire and Moléculaire-Génétique, Faculté de Médecine, Université des Sciences de la Santé, Libreville 241, Gabon
3
Department of Cellular and Molecular Medicine, Departement of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, the Panum Institute, University of Copenhagen, Copenhagen DK 2200N, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Jagdish Singh
Received: 30 September 2015 / Revised: 17 November 2015 / Accepted: 20 November 2015 / Published: 27 November 2015
(This article belongs to the Special Issue Cell-Penetrating Peptides)
View Full-Text   |   Download PDF [366 KB, uploaded 27 November 2015]   |  

Abstract

Chronic hepatitis B virus (HBV) infection remains a major health problem worldwide. Because current anti-HBV treatments are only virostatic, there is an urgent need for development of alternative antiviral approaches. In this context, cell-penetrating peptides (CPPs) and cationic polymers, such as chitosan (CS), appear of particular interest as nonviral vectors due to their capacity to facilitate cellular delivery of bioactive cargoes including peptide nucleic acids (PNAs) or DNA vaccines. We have investigated the ability of a PNA conjugated to different CPPs to inhibit the replication of duck hepatitis B virus (DHBV), a reference model for human HBV infection. The in vivo administration of PNA-CPP conjugates to neonatal ducklings showed that they reached the liver and inhibited DHBV replication. Interestingly, our results indicated also that a modified CPP (CatLip) alone, in the absence of its PNA cargo, was able to drastically inhibit late stages of DHBV replication. In the mouse model, conjugation of HBV DNA vaccine to modified CS (Man-CS-Phe) improved cellular and humoral responses to plasmid-encoded antigen. Moreover, other systems for gene delivery were investigated including CPP-modified CS and cationic nanoparticles. The results showed that these nonviral vectors considerably increased plasmid DNA uptake and expression. Collectively promising results obtained in preclinical studies suggest the usefulness of these safe delivery systems for the development of novel therapeutics against chronic hepatitis B. View Full-Text
Keywords: hepatitis B; antiviral therapy; cell penetrating peptides (CPPs); chitosan (CS); catonic polymers; peptide nucleic acids (PNAs); hepatitis B virus (HBV); duck hepatitis B virus (DHBV); DNA vaccine; gene delivery; antigen (Ag) hepatitis B; antiviral therapy; cell penetrating peptides (CPPs); chitosan (CS); catonic polymers; peptide nucleic acids (PNAs); hepatitis B virus (HBV); duck hepatitis B virus (DHBV); DNA vaccine; gene delivery; antigen (Ag)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ndeboko, B.; Lemamy, G.J.; Nielsen, P.E.; Cova, L. Therapeutic Potential of Cell Penetrating Peptides (CPPs) and Cationic Polymers for Chronic Hepatitis B. Int. J. Mol. Sci. 2015, 16, 28230-28241.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top