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Int. J. Mol. Sci. 2015, 16(11), 27625-27639; doi:10.3390/ijms161126046

Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers

1
Department of Chemical and Biomolecular Nanotechnology, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, C/Jordi Girona 18-26, 08034 Barcelona, Spain
2
Department of Food, Environmental and Nutritional Sciences (DEFENS), Division of Chemistry and Molecular Biology, University of Milan, Via Celoria 2, 20133 Milan, Italy
3
Department Institute of Molecular Science and Technologies, ISTM-CNR, Via G. Fantoli 16/15, 20138 Milan, Italy
4
IMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Asociada al Centro Nacional de Biotecnología (CSIC), C/Faraday 9, 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: O. Thompson Mefford
Received: 29 September 2015 / Revised: 5 November 2015 / Accepted: 6 November 2015 / Published: 18 November 2015
(This article belongs to the Special Issue Magnetic Nanoparticles 2015)
View Full-Text   |   Download PDF [1721 KB, uploaded 18 November 2015]   |  

Abstract

Au- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of α-thrombin to two aptamers conjugated to these NPs causes aggregation, a phenomenon that can be observed by UV, DLS and MRI. These techniques discriminate a single methylation in one of the aptamers, preventing aggregation due to the inability of α-thrombin to recognize it. A parallel study with gold and ferromagnetic NPs is detailed, concluding that the Au coating of FexOy NP does not affect their performance and that they are suitable as complex biosensors. These results prove the high detection potency of Au-coated SPIONs for biomedical applications especially for DNA repair detection. View Full-Text
Keywords: biosensor; gold-coated superparamagnetic nanoparticles; nanotechnology; aptamers biosensor; gold-coated superparamagnetic nanoparticles; nanotechnology; aptamers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tintoré, M.; Mazzini, S.; Polito, L.; Marelli, M.; Latorre, A.; Somoza, Á.; Aviñó, A.; Fàbrega, C.; Eritja, R. Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers. Int. J. Mol. Sci. 2015, 16, 27625-27639.

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