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Int. J. Mol. Sci. 2015, 16(11), 27520-27534; doi:10.3390/ijms161126042

Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai

1
Department of Fishery Biology, PuKyong National University, Busan 608-737, Korea
2
Department of Microbiology, PuKyong National University, Busan 608-737, Korea
3
Department of Marine and Fisheries Resources, Mokpo National University, Muan 534-729, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 17 September 2015 / Revised: 9 November 2015 / Accepted: 10 November 2015 / Published: 18 November 2015
(This article belongs to the Special Issue Fish Molecular Biology)
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Abstract

The Pacific abalone Haliotis discus hannai is used for commercial aquaculture in Korea. We examined the transcriptome of Pacific abalone Haliotis discus hannai siblings using NGS technology to identify genes associated with high growth rates. Pacific abalones grown for 200 days post-fertilization were divided into small-, medium-, and large-size groups with mean weights of 0.26 ± 0.09 g, 1.43 ± 0.405 g, and 5.24 ± 1.09 g, respectively. RNA isolated from the soft tissues of each group was subjected to RNA sequencing. Approximately 1%–3% of the transcripts were differentially expressed in abalones, depending on the growth rate. RT-PCR was carried out on thirty four genes selected to confirm the relative differences in expression detected by RNA sequencing. Six differentially-expressed genes were identified as associated with faster growth of the Pacific abalone. These include five up-regulated genes (including one specific to females) encoding transcripts homologous to incilarin A, perlucin, transforming growth factor-beta-induced protein immunoglobulin-heavy chain 3 (ig-h3), vitelline envelope zona pellucida domain 4, and defensin, and one down-regulated gene encoding tomoregulin in large abalones. Most of the transcripts were expressed predominantly in the hepatopancreas. The genes identified in this study will lead to development of markers for identification of high-growth-rate abalones and female abalones. View Full-Text
Keywords: pacific abalone; growth; differentially expressed genes pacific abalone; growth; differentially expressed genes
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Choi, M.-J.; Kim, G.-D.; Kim, J.-M.; Lim, H.K. Differentially-Expressed Genes Associated with Faster Growth of the Pacific Abalone, Haliotis discus hannai. Int. J. Mol. Sci. 2015, 16, 27520-27534.

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