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Int. J. Mol. Sci. 2015, 16(11), 27032-27043; doi:10.3390/ijms161126011

A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
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Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 25 September 2015 / Revised: 30 October 2015 / Accepted: 3 November 2015 / Published: 12 November 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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Abstract

A 1670 bp 5′-flanking region of the polyphenol oxidase (PPO) gene was isolated from red Swiss chard, a betalain-producing plant. This region, named promoter BvcPPOP, and its 5′-truncated versions were fused with the GUS gene and introduced into Arabidopsis, an anthocyanins-producing plant. GUS histochemical staining and quantitative analysis of transgenic plants at the vegetative and reproductive stages showed that BvcPPOP could direct GUS gene expression in vegetative organs with root- and petiole-preference, but not in reproductive organs including inflorescences shoot, inflorescences leaf, flower, pod and seed. This promoter was regulated by developmental stages in its driving strength, but not in expression pattern. It was also regulated by the abiotic stressors tested, positively by salicylic acid (SA) and methyl jasmonate (MeJA) but negatively by abscisic acid (ABA), gibberellin (GA), NaCl and OH. Its four 5′-truncated versions varied in the driving strength, but not obviously in expression pattern, and even the shortest version (−225 to +22) retained the root- and petiole- preference. This promoter is, to our knowledge, the first PPO promoter cloned and functionally elucidated from the betalain-producing plant, and thus provides not only a useful tool for expressing gene(s) of agricultural interest in vegetative organs, but also a clue to clarify the function of metabolism-specific PPO in betalain biosynthesis. View Full-Text
Keywords: anthocyanin-producing plant; betalain-producing plant; Beta vulgaris subsp. cicla; development stage; petiole-preference; polyphenol oxidase (PPO); promoter; root-preferential expression; vegetative organ anthocyanin-producing plant; betalain-producing plant; Beta vulgaris subsp. cicla; development stage; petiole-preference; polyphenol oxidase (PPO); promoter; root-preferential expression; vegetative organ
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yu, Z.-H.; Han, Y.-N.; Xiao, X.-G. A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants. Int. J. Mol. Sci. 2015, 16, 27032-27043.

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