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Int. J. Mol. Sci. 2015, 16(11), 26813-26831; doi:10.3390/ijms161125989

Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage

1
Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil
2
Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil
3
Department of Pathology, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil
4
Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos/SP 13566-590, Brazil
5
Hematology Hemotherapy Centre, Universidade de Campinas (UNICAMP), Campinas/SP 13083-878, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Francesc Cebrià
Received: 10 September 2015 / Revised: 12 October 2015 / Accepted: 15 October 2015 / Published: 9 November 2015
(This article belongs to the Special Issue Molecular and Cellular Basis of Regeneration and Tissue Repair)
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Abstract

Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score (8.3 ± 3.1), followed by the SCAFFOLD group (5.6 ± 2.2) and the EMPTY group (5.2 ± 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model. View Full-Text
Keywords: tissue engineering; regenerative medicine; adult stem cell; stem cell therapy; biomaterials; cartilage tissue engineering; regenerative medicine; adult stem cell; stem cell therapy; biomaterials; cartilage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zorzi, A.R.; Amstalden, E.M.I.; Plepis, A.M.G.; Martins, V.C.A.; Ferretti, M.; Antonioli, E.; Duarte, A.S.S.; Luzo, A.C.M.; Miranda, J.B. Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage. Int. J. Mol. Sci. 2015, 16, 26813-26831.

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