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Int. J. Mol. Sci. 2015, 16(11), 26667-26676; doi:10.3390/ijms161125986

Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells

Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjyo, Kumamoto 860-8556, Japan
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Academic Editor: Jagdish Singh
Received: 30 September 2015 / Revised: 23 October 2015 / Accepted: 30 October 2015 / Published: 6 November 2015
(This article belongs to the Special Issue Cell-Penetrating Peptides)
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Abstract

Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdx1, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies. View Full-Text
Keywords: cell-penetrating peptide; poly-arginine; protein transduction; induced pluripotent stem cell; pancreatic differentiation cell-penetrating peptide; poly-arginine; protein transduction; induced pluripotent stem cell; pancreatic differentiation
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Kaitsuka, T.; Tomizawa, K. Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells. Int. J. Mol. Sci. 2015, 16, 26667-26676.

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