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Int. J. Mol. Sci. 2015, 16(11), 26211-26236; doi:10.3390/ijms161125954

Pooling and Analysis of Published in Vitro Data: A Proof of Concept Study for the Grouping of Nanoparticles

Health & Environment Department, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, Tulln 3430, Austria
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 6 July 2015 / Revised: 23 September 2015 / Accepted: 20 October 2015 / Published: 2 November 2015
(This article belongs to the Collection Bioactive Nanoparticles)
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Abstract

The study aim was to test the applicability of pooling of nanomaterials-induced in vitro data for identifying the toxic capacity of specific (SiO2, TiO2, ZnO, CuO, CeO2 and carbon nanotubes, [CNT]) nanoparticles (NP) and to test the usefulness for grouping purposes. Publication selection was based on specific criteria regarding experimental conditions. Two relevant biological endpoints were selected; generation of intracellular reactive oxygen species (ROS) and viability above 90%. The correlations of the ROS ratios with the NP parameters’ size, concentration, and exposure time were analysed. The obtained data sets were then analysed with multiple regression analysis of variance (ANOVA) and the Tukey post-hoc test. The results show that this method is applicable for the selected metal oxide NP, but might need reconsideration and a larger data set for CNT. Several statistically significant correlations and results were obtained, thus validating the method. Furthermore, the relevance of the combination of ROS release with a cell viability test was shown. The data also show that it is advisable to compare ROS production of professional phagocytic with non-phagocytic cells. In conclusion, this is the first systematic analysis showing that pooling of available data into groups is a useful method for evaluation of data regarding NP induced toxicity in vitro. View Full-Text
Keywords: nanoparticles; toxicity; intracellular ROS; cell viability; phagocytic and non-phagocytic cells; CNT nanoparticles; toxicity; intracellular ROS; cell viability; phagocytic and non-phagocytic cells; CNT
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Simkó, M.; Tischler, S.; Mattsson, M.-O. Pooling and Analysis of Published in Vitro Data: A Proof of Concept Study for the Grouping of Nanoparticles. Int. J. Mol. Sci. 2015, 16, 26211-26236.

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