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Int. J. Mol. Sci. 2015, 16(11), 26077-26086; doi:10.3390/ijms161125950

Application of CRISPR/Cas9 Technology to HBV

National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China
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Author to whom correspondence should be addressed.
Academic Editor: Izuho Hatada
Received: 8 September 2015 / Revised: 25 October 2015 / Accepted: 26 October 2015 / Published: 2 November 2015
(This article belongs to the Special Issue Genome Editing)
View Full-Text   |   Download PDF [893 KB, uploaded 2 November 2015]   |  

Abstract

More than 240 million people around the world are chronically infected with hepatitis B virus (HBV). Nucleos(t)ide analogs and interferon are the only two families of drugs to treat HBV currently. However, none of these anti-virals directly target the stable nuclear covalently closed circular DNA (cccDNA), which acts as a transcription template for viral mRNA and pre-genomic RNA synthesis and secures virus persistence. Thus, the fact that only a small number of patients treated achieve sustained viral response (SVR) or cure, highlights the need for new therapies against HBV. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system can specifically target the conserved regions of the HBV genome. This results in robust viral suppression and provides a promising tool for eradicating the virus. In this review, we discuss the function and application of the CRISPR/Cas9 system as a novel therapy for HBV. View Full-Text
Keywords: CRISPR/Cas9; HBV; cccDNA; antiviral CRISPR/Cas9; HBV; cccDNA; antiviral
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lin, G.; Zhang, K.; Li, J. Application of CRISPR/Cas9 Technology to HBV. Int. J. Mol. Sci. 2015, 16, 26077-26086.

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