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Int. J. Mol. Sci. 2015, 16(10), 25141-25153; doi:10.3390/ijms161025141

Detection and Identification of Probiotic Lactobacillus plantarum Strains by Multiplex PCR Using RAPD-Derived Primers

1
Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece
2
Institute of Technology of Agricultural Products, Hellenic Agricultural Organization-DEMETER, Sof. Venizelou 1, Lycovrissi, Attiki 14123, Greece
*
Authors to whom correspondence should be addressed.
Academic Editor: Alejandro Cifuentes
Received: 9 September 2015 / Revised: 2 October 2015 / Accepted: 16 October 2015 / Published: 22 October 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Lactobacillus plantarum 2035 and Lactobacillus plantarum ACA-DC 2640 are two lactic acid bacteria (LAB) strains that have been isolated from Feta cheese. Both display significant potential for the production of novel probiotic food products. The aim of the present study was the development of an accurate and efficient method for the molecular detection and identification of the above strains in a single reaction. A multiplex PCR assay was designed for each strain, based on specific primers derived from Random Amplified Polymorphic DNA (RAPD) Sequenced Characterized Amplified Region (SCAR) analysis. The specificity of the assay was tested with a total of 23 different LAB strains, for L. plantarum 2035 and L. plantarum ACA-DC 2640. The multiplex PCR assay was also successfully applied for the detection of the above cultures in yogurt samples prepared in our lab. The proposed methodology may be applied for monitoring the presence of these strains in food products, thus evaluating their probiotic character. Moreover, our strategy may be adapted for other novel LAB strains with probiotic potential, thus providing a powerful tool for molecular discrimination that could be invaluable to the food industry. View Full-Text
Keywords: probiotics; lactic acid bacteria; Lactobacillus plantarum; multiplex PCR; RAPD; SCAR probiotics; lactic acid bacteria; Lactobacillus plantarum; multiplex PCR; RAPD; SCAR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Galanis, A.; Kourkoutas, Y.; Tassou, C.C.; Chorianopoulos, N. Detection and Identification of Probiotic Lactobacillus plantarum Strains by Multiplex PCR Using RAPD-Derived Primers. Int. J. Mol. Sci. 2015, 16, 25141-25153.

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