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Int. J. Mol. Sci. 2015, 16(10), 25121-25140; doi:10.3390/ijms161025121

Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation

1
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
2
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
3
Plant Protection Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
4
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA
5
Department of Crop Biotechnology, Agronomy College, Henan Agricultural University, Zhengzhou 450002, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Jan Schirawski
Received: 27 June 2015 / Revised: 12 October 2015 / Accepted: 12 October 2015 / Published: 22 October 2015
(This article belongs to the Special Issue Plant Microbe Interaction)
View Full-Text   |   Download PDF [1533 KB, uploaded 22 October 2015]   |  

Abstract

Verticillium wilt is threatening cotton productivity globally. This disease is caused by soil-borne Verticillium dahliae which directly infects cotton roots, and exclusively colonizes and occludes xylem vessels, finally resulting in necrosis, defoliation, and most severely, plant death. For the first time, iTRAQ (isobaric tags for relative and absolute quantification) was applied to screen the differentially expressed proteins of Gossypium thurberi inoculated with V. dahliae. A total of 6533 proteins were identified from the roots of G. thurberi after inoculation with V. dahliae, and 396 showed up- and 279 down-regulated in comparison to a mock-inoculated roots. Of these identified proteins, the main functional groups were those involved in cell wall organization and reinforcement, disease-resistant chemicals of secondary metabolism, phytohormone signaling, pathogenesis-related proteins, and disease-resistant proteins. Physiological and biochemical analysis showed that peroxidase activity, which promotes the biosynthesis and accumulation of lignin, was induced early in the hypocotyl after inoculation with V. dahliae. Similarly, salicylic acid also accumulated significantly in hypocotyl of the seedlings after inoculation. These findings provide an important knowledge of the molecular events and regulatory networks occurring during G. thurberi-V. dahliae interaction, which may provide a foundation for breeding disease-resistance in cotton. View Full-Text
Keywords: cotton; defense response; fungus; iTRAQ cotton; defense response; fungus; iTRAQ
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fang, W.; Xie, D.; Zhu, H.; Li, W.; Xu, Z.; Yang, L.; Li, Z.; Sun, L.; Wang, J.; Nie, L.; Tang, Z.; Lv, S.; Zhao, F.; Sun, Y.; Zhao, Y.; Hou, J.; Yang, X. Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation. Int. J. Mol. Sci. 2015, 16, 25121-25140.

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