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Int. J. Mol. Sci. 2015, 16(10), 24918-24945; doi:10.3390/ijms161024918

High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives

Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands
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Academic Editor: Qiang “Shawn” Chen
Received: 14 August 2015 / Revised: 13 October 2015 / Accepted: 13 October 2015 / Published: 20 October 2015
(This article belongs to the Special Issue Protein Engineering)
View Full-Text   |   Download PDF [1831 KB, uploaded 20 October 2015]   |  

Abstract

Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms. View Full-Text
Keywords: high-throughput screening; FACS-based screening platforms; microfluidics-based screening platforms; protein engineering; in vitro compartmentalization high-throughput screening; FACS-based screening platforms; microfluidics-based screening platforms; protein engineering; in vitro compartmentalization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wójcik, M.; Telzerow, A.; Quax, W.J.; Boersma, Y.L. High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives. Int. J. Mol. Sci. 2015, 16, 24918-24945.

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