Next Article in Journal
Medullary Thymic Epithelial Cells and Central Tolerance in Autoimmune Hepatitis Development: Novel Perspective from a New Mouse Model
Next Article in Special Issue
Trans-Membrane Area Asymmetry Controls the Shape of Cellular Organelles
Previous Article in Journal
BRD4 Inhibitor Inhibits Colorectal Cancer Growth and Metastasis
Previous Article in Special Issue
Membrane Trafficking in the Yeast Saccharomyces cerevisiae Model
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2015, 16(1), 1949-1979; doi:10.3390/ijms16011949

Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches

1
National Enterprise for nanoScience and nanoTechnology (NEST) Laboratory, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, Pisa I-56127, Italy
2
Biology Laboratory (BioSNS), Scuola Normale Superiore and Istituto di Neuroscienze-CNR, via Moruzzi 1, Pisa I-56100, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Jeremy C. Simpson
Received: 30 November 2014 / Accepted: 5 January 2015 / Published: 16 January 2015
View Full-Text   |   Download PDF [883 KB, uploaded 16 January 2015]   |  

Abstract

Neurotrophins are secreted proteins that regulate neuronal development and survival, as well as maintenance and plasticity of the adult nervous system. The biological activity of neurotrophins stems from their binding to two membrane receptor types, the tropomyosin receptor kinase and the p75 neurotrophin receptors (NRs). The intracellular signalling cascades thereby activated have been extensively investigated. Nevertheless, a comprehensive description of the ligand-induced nanoscale details of NRs dynamics and interactions spanning from the initial lateral movements triggered at the plasma membrane to the internalization and transport processes is still missing. Recent advances in high spatio-temporal resolution imaging techniques have yielded new insight on the dynamics of NRs upon ligand binding. Here we discuss requirements, potential and practical implementation of these novel approaches for the study of neurotrophin trafficking and signalling, in the framework of current knowledge available also for other ligand-receptor systems. We shall especially highlight the correlation between the receptor dynamics activated by different neurotrophins and the respective signalling outcome, as recently revealed by single-molecule tracking of NRs in living neuronal cells. View Full-Text
Keywords: neurotrophin receptors; nerve growth factor; single molecule imaging; single molecule tracking; single molecule interactions; binding stoichiometry; receptor clustering; membrane trafficking; receptor internalization neurotrophin receptors; nerve growth factor; single molecule imaging; single molecule tracking; single molecule interactions; binding stoichiometry; receptor clustering; membrane trafficking; receptor internalization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Marchetti, L.; Luin, S.; Bonsignore, F.; de Nadai, T.; Beltram, F.; Cattaneo, A. Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches. Int. J. Mol. Sci. 2015, 16, 1949-1979.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top