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Int. J. Mol. Sci. 2015, 16(1), 1562-1575; doi:10.3390/ijms16011562

Rapid and Sensitive Identification of the Herbal Tea Ingredient Taraxacum formosanum Using Loop-Mediated Isothermal Amplification

1
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan
2
Department & Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan
3
Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan
4
Department of Bioresources, Da-Yeh University, Changhua 51591, Taiwan
5
Management Center, Department of Medical Research and Development, Show Chwan Health Care Sysytem, Changhua 51951, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 31 October 2014 / Accepted: 31 December 2014 / Published: 9 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1597 KB, uploaded 9 January 2015]   |  

Abstract

Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control. View Full-Text
Keywords: Taraxacum formosanum; medicinal plant; internal transcribed spacers 2 (ITS2); loop-mediated isothermal amplification (LAMP); rapid authentication; adulterant; quality control Taraxacum formosanum; medicinal plant; internal transcribed spacers 2 (ITS2); loop-mediated isothermal amplification (LAMP); rapid authentication; adulterant; quality control
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lai, G.-H.; Chao, J.; Lin, M.-K.; Chang, W.-T.; Peng, W.-H.; Sun, F.-C.; Lee, M.-S.; Lee, M.-S. Rapid and Sensitive Identification of the Herbal Tea Ingredient Taraxacum formosanum Using Loop-Mediated Isothermal Amplification. Int. J. Mol. Sci. 2015, 16, 1562-1575.

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