Hydrostatic Pressure Influences HIF-2 Alpha Expression in Chondrocytes
AbstractHypoxia-inducible factor (HIF)-2α is considered to play a major role in the progression of osteoarthritis. Recently, it was reported that pressure amplitude influences HIF-2α expression in murine endothelial cells. We examined whether hydrostatic pressure is involved in expression of HIF-2α in articular chondrocytes. Chondrocytes were cultured and stimulated by inflammation or hydrostatic pressure of 0, 5, 10, or 50 MPa. After stimulation, heat shock protein (HSP) 70, HIF-2α, nuclear factor kappa B (NF-κB), matrix metalloproteinase (MMP)-13, MMP-3, and vascular endothelial growth factor (VEGF) gene expression were evaluated. The levels of all gene expression were increased by inflammatory stress. When chondrocytes were exposed to a hydrostatic pressure of 5 MPa, HIF-2α, MMP-13, and MMP-3 gene expression increased significantly although those of HSP70 and NF-κB were not significantly different from the control group. In contrast, HIF-2α gene expression did not increase under a hydrostatic pressure of 50 MPa although HSP70 and NF-κB expression increased significantly compared to control. We considered that hydrostatic pressure of 5 MPa could regulate HIF-2α independent of NF-κB, because the level of HIF-2α gene expression increased significantly without upregulation of NF-κB expression at 5 MPa. Hydrostatic pressure may influence cartilage degeneration, inducing MMP-13 and MMP-3 expression through HIF-2α. View Full-Text
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Inoue, H.; Arai, Y.; Kishida, T.; Terauchi, R.; Honjo, K.; Nakagawa, S.; Tsuchida, S.; Matsuki, T.; Ueshima, K.; Fujiwara, H.; Mazda, O.; Kubo, T. Hydrostatic Pressure Influences HIF-2 Alpha Expression in Chondrocytes. Int. J. Mol. Sci. 2015, 16, 1043-1050.
Inoue H, Arai Y, Kishida T, Terauchi R, Honjo K, Nakagawa S, Tsuchida S, Matsuki T, Ueshima K, Fujiwara H, Mazda O, Kubo T. Hydrostatic Pressure Influences HIF-2 Alpha Expression in Chondrocytes. International Journal of Molecular Sciences. 2015; 16(1):1043-1050.Chicago/Turabian Style
Inoue, Hiroaki; Arai, Yuji; Kishida, Tsunao; Terauchi, Ryu; Honjo, Kuniaki; Nakagawa, Shuji; Tsuchida, Shinji; Matsuki, Tomohiro; Ueshima, Keiichirou; Fujiwara, Hiroyoshi; Mazda, Osam; Kubo, Toshikazu. 2015. "Hydrostatic Pressure Influences HIF-2 Alpha Expression in Chondrocytes." Int. J. Mol. Sci. 16, no. 1: 1043-1050.