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Int. J. Mol. Sci. 2014, 15(9), 16665-16679; doi:10.3390/ijms150916665

Inhibitory Effects of Adlay Extract on Melanin Production and Cellular Oxygen Stress in B16F10 Melanoma Cells

1
Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
2
Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu Dist., Taichung City 43302, Taiwan
3
Niuer International Skincare Science Research Institute, 7F, No. 618, Ruiguang Rd., Neihu Dist., Taipei 114, Taiwan
*
Author to whom correspondence should be addressed.
Received: 6 January 2014 / Revised: 12 September 2014 / Accepted: 15 September 2014 / Published: 19 September 2014
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Abstract

The aim of this study was to determine the effects of adlay extract on melanin production and the antioxidant characteristics of the extract. The seeds were extracted by the supercritical fluid CO2 extraction (SFE) method. The effect of adlay extract on melanin production was evaluated using mushroom tyrosinase activity assay, intracellular tyrosinase activity, antioxidant properties and melanin content. Those assays were performed spectrophotometrically. In addition, the expression of melanogenesis-related proteins was determined by western blotting. The results revealed that the adlay extract suppressed intracellular tyrosinase activity and decreased the amount of melanin in B16F10 cells. The adlay extract decreased the expression of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2). The extract also exhibited antioxidant characteristics such as free radical scavenging capacity and reducing power. It effectively decreased intracellular reactive oxygen species (ROS) levels in B16F10 cells. We concluded that the adlay extract inhibits melanin production by down-regulation of MITF, tyrosinase, TRP-1 and TRP-2. The antioxidant properties of the extract may also contribute to the inhibition of melanogenesis. The adlay extract can therefore be applied as an inhibitor of melanogenesis and could also act as a natural antioxidant in skin care products. View Full-Text
Keywords: adlay; MITF; tyrosinase; melanin; ROS adlay; MITF; tyrosinase; melanin; ROS
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MDPI and ACS Style

Huang, H.-C.; Hsieh, W.-Y.; Niu, Y.-L.; Chang, T.-M. Inhibitory Effects of Adlay Extract on Melanin Production and Cellular Oxygen Stress in B16F10 Melanoma Cells. Int. J. Mol. Sci. 2014, 15, 16665-16679.

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